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黄方(教授)
发布时间: 2017-03-05  作者:  浏览次数: 3588

 

»姓名: 黄方

»职称:教授/博导

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»系属:生物工程与技术中心

»最高学位:博士

»学科:生物化工

»所学专业:化学

»电子邮箱:fhuang@upc.edu.cn

»联系电话: 0532-86981560

»地址邮编:山东省青岛市黄岛区长江西路66号中国石油大学(华东)化学工程学院,266580

»个人主页:http://pme.upc.edu.cn/

学习与工作经历

1991.9-1995.7

山东大学,化学院,学士学位

1997.9-2000.7

山东大学,化学院,硕士学位

2000.8-2004.6

瑞士巴塞尔大学,物理化学研究所,博士(Prof. Werner Nau

2004.7-2008.6

英国剑桥大学MRC蛋白质工程中心,博士后(Sir Alan Fersht

2008.6-2009.9

英国剑桥大学MRC蛋白质工程中心,科学家(Investigator Scientist),长期职位(Established

2009.6-

中国石油大学生物工程与技术中心,教授,博士生导师


研究方向
蛋白质工程、单分子荧光、生物能源、生物材料
学术兼职

中国化学会生物物理化学专业委员会委员

中国生物物理学会单分子生物学分会理事

山东省物理学会生物物理专业委员会副主任委员
主讲课程
蛋白质工程、高等生物化学、生命科学与生物技术进展
指导研究生
每年招收1-2名博士研究生、2-4名硕士研究生
承担科研课题
2011-2013
年承担国家自然科学基金面上项目“菌视紫红质去折叠态和折叠中间态的单分子研究”

2011-2014年承担国家自然科学基金重点项目“多孔薄膜和溶液聚集体结构自组装、理论模拟与性能的基础研究”;

2012-2016年承担国家973项目“重大心血管疾病相关GPCR新药物靶点的基础研究”;

2013-2016年年承担国家自然科学基金项目“单螺旋跨膜多肽在生物膜中取向的研究”;

2016-2019年承担国家自然科学基金项目“多态蛋白状态转变的单分子荧光研究”
获奖情况

2012年,山东青年五四奖章


荣誉称号
泰山学者海外特聘专家;山东省自然科学杰出青年基金获得者;教育部新世纪优秀人才支持计划入选者
著作

论文

1.   Zhao Y, et al. (2016) Stable folding intermediates prevent fast interconversion between the closed and open states of Mad2 through its denatured state. Protein engineering, design & selection : PEDS 29(1):23-29.

2.   Yue T, Xu Y, Sun M, Zhang X, & Huang F (2016) How tubular aggregates interact with biomembranes: wrapping, fusion and pearling. Phys. Chem. Chem. Phys. 18(2):1082-1091.

3.   Yue T, Xu Y, Li S, Zhang X, & Huang F (2016) Lipid extraction mediates aggregation of carbon nanospheres in pulmonary surfactant monolayers. Phys. Chem. Chem. Phys. 18(28):18923-18933.

4.   Yue T, Tian F, Sun M, Zhang X, & Huang F (2016) Inter-tube adhesion mediates a new pearling mechanism. Phys. Chem. Chem. Phys. 18(1):361-374.

5.   Yue T, et al. (2016) How transmembrane peptides insert and orientate in biomembranes: a combined experimental and simulation study. Phys. Chem. Chem. Phys. 18(26):17483-17494.

6.   Yue T, Li S, Xu Y, Zhang X, & Huang F (2016) Interplay Between Nanoparticle Wrapping and Clustering of Inner Anchored Membrane Proteins. J. Phys. Chem. B.

7.   Wang XJ, et al. (2016) Steering graphene quantum dots in living cells: lighting up the nucleolus. J Mater Chem B 4(4):779-784.

8.   Wang X, et al. (2016) Active tumor-targeting luminescent gold clusters with efficient urinary excretion. Chem Commun (Camb) 52(59):9232-9235.

9.   He H, et al. (2016) An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chemistry 22(17):5863-5867.

10.  Chi H, Wang X, Li J, Ren H, & Huang F (2016) Chaperonin-enhanced Escherichia coli cell-free expression of functional CXCR4. J. Biotechnol. 231:193-200.

11.  Yue TT, Wang XJ, Zhang XR, & Huang F (2015) Molecular modeling of interaction between lipid monolayer and graphene nanosheets: implications for pulmonary nanotoxicity and pulmonary drug delivery. Rsc Adv 5(38):30092-30106.

12.  Yue T, Zhang X, & Huang F (2015) Molecular modeling of membrane responses to the adsorption of rotating nanoparticles: promoted cell uptake and mechanical membrane rupture. Soft matter 11(3):456-465.

13.  Yu D, et al. (2015) Enhanced photocurrent production by bio-dyes of photosynthetic macromolecules on designed TiO2 film. Scientific reports 5:9375.

14.  Wang X, et al. (2015) A two-component active targeting theranostic agent based on graphene quantum dots. J. Mater. Chem. B 3:3583--3590.

15.  Wang X, Huang H, Sun C, & Huang F (2015) Structural analysis of bacteriorhodopsin solubilized by lipid-like phosphocholine biosurfactants with varying micelle concentrations. J. Colloid Interface Sci. 437:170-180.

16.  Ge BS, Wang MQ, Li JQ, Liu JG, & Huang F (2015) Maltose binding protein facilitates functional production of engineered human chemokine receptor 3 in Escherichia coli. Process Biochem. 50(2):285-293.

17.  Chi H, Wang X, Li J, Ren H, & Huang F (2015) Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES. Scientific reports 5:17037.

18.  Yue T, Zhang X, & Huang F (2014) Membrane monolayer protrusion mediates a new nanoparticle wrapping pathway. Soft matter 10(12):2024-2034.

19.  Yue T, Zhang X, & Huang F (2014) Molecular modeling of membrane tube pearling and the effect of nanoparticle adsorption. Phys. Chem. Chem. Phys. 16(22):10799-10809.

20.  Yue T, Zhang X, & Huang F (2014) Membrane monolayer protrusion mediates a new nanoparticle wrapping pathway. Soft matter 10(12):2024-2034.

21.  Yu D, et al. (2014) Triton X-100 as an effective surfactant for the isolation and purification of photosystem I from Arthrospira platensis. Photosynth. Res. 120(3):311-321.

22.  Yu D, et al. (2014) Effect of surfactants on apparent oxygen consumption of photosystem I isolated from Arthrospira platensis. Photosynth. Res. 122(2):203-213.

23.  Wang X, et al. (2014) Multifunctional graphene quantum dots for simultaneous targeted cellular imaging and drug delivery. Colloids and surfaces. B, Biointerfaces 122:638-644.

24.  Wang M, Ge B, Yang Q, Jiang X, & Huang F (2014) High-level production of biologically active chemokines in Escherichia coli. Process Biochem. 49(4):706-714.

25.  Song B, et al. (2014) Irreversible Denaturation of Proteins through Aluminum-Induced Formation of Backbone Ring Structures. Angew. Chem. Int. Ed. 53(25):6358-6363.

26.  Qu JB, et al. (2014) Coating gigaporous polystyrene microspheres with cross-linked poly(vinyl alcohol) hydrogel as a rapid protein chromatography matrix. ACS applied materials & interfaces 6(15):12752-12760.

27.  Yue T, Wang X, Huang F, & Zhang X (2013) An unusual pathway for the membrane wrapping of rodlike nanoparticles and the orientation- and membrane wrapping-dependent nanoparticle interaction. Nanoscale 5(20):9888-9896.

28.  Yu D, Zhu G, Liu S, Ge B, & Huang F (2013) Photocurrent activity of light-harvesting complex II isolated from spinach and its pigments in dye-sensitized TiO2 solar cell. Int. J. Hydrogen Energy 38(36):16740-16748.

29.  Wang X, et al. (2013) Solubilization and Stabilization of Isolated Photosystem I Complex with Lipopeptide Detergents. PLoS One 8(9):e76256.

30.  Wang M, et al. (2013) Milligram Production and Biological Activity Characterization of the Human Chemokine Receptor CCR3. PLoS One 8(6):e65500.

31.  Qu JB, Shao HH, Jing GL, & Huang F (2013) PEG-chitosan-coated iron oxide nanoparticles with high saturated magnetization as carriers of 10-hydroxycamptothecin: preparation, characterization and cytotoxicity studies. Colloids and surfaces. B, Biointerfaces 102:37-44.

32.  Huang F, Johnson CM, Petrovich M, & Fersht AR (2013) Don't waste good methods on bad buffers and ambiguous data. Proc Natl Acad Sci U S A 110(5):E331-332.

33.  Ge B, et al. (2013) Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli. Biotechnol. Lett 35(5):689-693.

34.  Yu D, Huang F, & Xu H (2012) Determination of critical concentrations by synchronous fluorescence spectrometry. Analytical Methods 4(1):47-49.

35.  Leith JS, et al. (2012) Sequence-dependent sliding kinetics of p53. Proc Natl Acad Sci U S A 109(41):16552-16557.

36.  Tafvizi A, Huang F, Fersht AR, Mirny LA, & van Oijen AM (2011) A single-molecule characterization of p53 search on DNA. Proc Natl Acad Sci U S A 108(2):563-568.

37.  Rajagopalan S, Huang F, & Fersht AR (2011) Single-Molecule characterization of oligomerization kinetics and equilibria of the tumor suppressor p53. Nucleic Acids Res. 39(6):2294-2303.

38.  Huang F, Ying L, Neuweiler H, & Fersht AR (2009) Reply to Campos et al.: Direct observation versus ambiguous kinetics and thermodynamics. Proce Natl Acad Sci USA 106(52):DOI: 10.1073/pnas.0913321107.

39.  Huang F, Ying L, & Fersht AR (2009) Direct observation of barrier-limited folding of BBL by single-molecule fluorescence resonance energy transfer. Proc Natl Acad Sci USA 106(38):16239-16244.

40.  Huang F, et al. (2009) Multiple conformations of full-length p53 detected with single-molecule fluorescence resonance energy transfer. Proc Natl Acad Sci USA 106:20758-20763.

41.  Huang F, et al. (2009) Time-resolved fluorescence resonance energy transfer study shows a compact denatured state of the B domain of protein A. Biochemistry 48(15):3468-3476.

42.  Tafvizi A, et al. (2008) Tumor suppressor p53 slides on DNA with low friction and high stability. Biophys. J. 95(1):L01-L03.

43.  Huang F, Settanni G, & Fersht AR (2008) Fluorescence resonance energy transfer analysis of the folding pathway of Engrailed Homeodomain. Protein engineering, design & selection : PEDS 21(3):131-146.

44.  Huang F, Sato S, Sharpe TD, Ying L, & Fersht AR (2007) Distinguishing between cooperative and unimodal downhill protein folding. Proc Natl Acad Sci USA 104(1):123-127.

45.  Huang F & Nau WM (2005) Photochemical techniques for studying the flexibility of polypeptides. Res. Chem. Intermed. 31(7-8):717-726.

46.  Pischel U, Huang F, & Nau WM (2004) Intramolecular singlet-singlet energy transfer in antenna-substituted azoalkanes. Photochem Photobiol Sci 3(3):305-310.

47.  Marquez C, Huang F, & Nau WM (2004) Cucurbiturils: molecular nanocapsules for time-resolved fluorescence-based assays. IEEE Trans Nanobioscience 3(1):39-45.

48.  Huang F, Hudgins RR, & Nau WM (2004) Primary and secondary structure dependence of peptide flexibility assessed by fluorescence-based measurement of end-to-end collision rates. J. Am. Chem. Soc. 126(50):16665-16675.

49.  Nau WM, et al. (2003) Exploiting long-lived molecular fluorescence. Chimia 57(4):161-167.

50.  Huang F & Nau WM (2003) A conformational flexibility scale for amino acids in peptides. Angew. Chem. Int. Ed. 42(20):2269-2272.

51.  Gao X, et al. (2003) Progresses of derivatization techniques for analyses of carbohydrates. Anal. Lett. 36(7):1281-1310.

52.  Yang J, Huang F, Wang M, Wu X, & Sun C (2002) Study on the molecular recognitions of calix[n]arenes to 2,3-diaminonaphthalene by using fluorometric technique. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 58(8):1775-1778.

53.  Liu R, et al. (2002) Resonance Rayleigh light-scattering of thiamine hydrochloride-nucleic acids-cetyl trimethyl ammonium bromide systems and their analytical application. J. Trace Microprobe Tech. 20(3):363-376.

54.  Hudgins RR, Huang F, Gramlich G, & Nau WM (2002) A fluorescence-based method for direct measurement of submicrosecond intramolecular contact formation in biopolymers: an exploratory study with polypeptides. J. Am. Chem. Soc. 124(4):556-564.

55.  Huang F, et al. (2001) The study of spectrosgraphic properties and molecular recognition of cali. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 57A(5):1025-1030.

56.  Yang J, Cao X, Huang F, Wu X, & Zhou G (2000) Fluorimetric determination of carbohydrates with 2,3-diaminonaphthalene. Chem. J. Internet 2(1).

57.  Wu X, et al. (2000) A study on the fluorescence system of Sc-BPMPHD-CTMAB and its analytical application. Chem. Res. Chin. Uni. 16(1):93-95.

58.  Wang M, Yang J, Wu X, & Huang F (2000) Study of the interaction of nucleic acids with acridine red and CTMAB by a resonance light scattering technique and determination of nucleic acids at nanogram levels. Anal. Chim. Acta 422(2):151-158.

59.  Ma Q, Yang J, Wu X, Huang F, & Sun L (2000) A selective fluorimetric method for the determination of some b-lactamic antibiotics. Anal. Lett. 33(13):2689-2699.

60.  Huang F, Liu X, Zhou G, Wu X, & Yang J (2000) The progress of the derivatization methods of carbohydrate. Chem. Online 2000(1-12):c00023.

61.  Wu X, et al. (1999) Study of the reaction between nucleic acids and Tb-BPMPHD-CTMAB complex and its analytical application. Anal. Lett. 32(12):2417-2425.

62.  Wu X, Tong C, Su B, Huang F, & Yang J (1999) Determination of dopamine by fluorimetry. Chin. J. Anal. Chem. 27(9):1069-1071.

63.  Wu X, Tong C, Su B, Huang F, & Yang J (1999) Terbium(III) as fluorescence probe for the determination of epinephrine. Shandong Daxue Xuebao, Ziran Kexueban 34(3):352-355.

64.  Yang J, et al. (1998) Fluorometric determination of epinephrine with o-phenylenediamine. Anal. Chim. Acta 363(1):105-110.

65.  Yang J, Ma Q, Huang F, Sun L, & Dong J (1998) A new fluorimetric method for the determination of ascorbic acid. Anal. Lett. 31(15):2757-2766.

 

专利

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地址:山东省青岛市黄岛区长江西路66号