何 化（副教授）

»姓名： 何 化

»职称：副教授/硕导

系属：生物工程与技术中心

»最高学位：博士

»学科：生物工程、生物化工

»所学专业：分析化学、应用化学

»电子邮箱：huahe@upc.edu.cn

»联系电话：18562789926/0532-86981135

»地址邮编：山东省青岛市黄岛区长江西路66号中国石油大学（华东）化学工程学院,266580

»个人主页：http://cbb.cce.upc.edu.cn/2018/1202/c12413a177424/page.htm

◎学习与工作经历

2008/12-至今 中国石油大学（华东）化学工程学院生物中心；

2014/02-2015/02   美国斯坦福大学化学系国家公派留学，师从美国科学院院士Hongjie Dai（戴宏杰)教授；

2005/09-2008/11   上海交通大学应用化学专业工学博士，师从任吉存教授；

2002/9-2005/7湖南师范大学分析化学专业理学硕士，师从中国科学院院士姚守拙教授；

1998/9-2002/7   湖南师范大学应用化学专业理学学士学位。
◎研究方向
1)   单分子超分辨荧光成像、高深度近红外荧光成像、表面增强荧光成像等新成像技术的开发

2)   GPCR等药物靶点分子信号转导机制、活体生物成像诊断和体外肿瘤标志物高灵敏检测

3)   分子影像探针（有机小分子和荧光纳米粒子）的合成

◎学术兼职

中国化学会会员；ACS   Nano, ACS Appl. Mater. Interfaces, J. Mater. Chem. B等国际期刊特邀审稿人。
◎主讲课程
本科生课程《生物工程概论》、《材料与环境》；研究生课程《生物材料》、《生命科学与生物技术进展》；面向留学生的全英文课程《现代仪器分析技术》。
◎指导研究生
指导和协助指导博士研究生、国外留学博士生、硕士研究生和本科毕业生。其中，1人获山东省优秀本科毕业论文。。
◎主持科研项目

1）  国家自然科学基金面上项目（21874154），《细胞膜上G蛋白偶联受体聚集与分布的荧光闪烁碳点超分辨成像》；

2）  国家自然科学基金青年项目（20905078），《基于RGD配体设计的CdTe量子点靶向探针的直接合成及其应用》；

3）  山东省自然基金面上项目（ZR2014BM028），《荧光闪烁量子点用于细胞中趋化因子受体CCR3的三维超分辨定位与分布研究》；

4）  山东省自然基金青年项目（ZR2009BQ001），《基于RGD肽的量子点靶向探针制备新方法研究及应用》。

5）  中央高校基本科研业务基金（18CX02126A），《荧光碳纳米材料的制备及其在生物成像中的应用》。

6）  中央高校基本科研业务基金（12CX04053A），《单个量子点的光学行为、调控及其应用研究》。
◎获奖情况

2018年青岛市自然科学奖三等奖（排名第5）

国家优秀奖学金、香港求是研究生奖学金、“上海交大优秀毕业生”

◎以第一作者/通讯作者发表的论文论著：

  在Angew. Chem. Int. Ed., Anal. Chem., Chem. Commun., ACS Appl.   Mater. Interfaces 等国际权威SCI刊物发表论文18篇，受邀撰写Springer出版社英文专著1章。其中，中科院JCR一区8篇，二区5篇，总引用698次，单篇最高被引用206次。

1.            Hua   He,   Xiaojuan Wang, Fang Huang. Analysis and Applications of Single Molecule   Fluorescence in Live Cell Membranes, in: ‎Membrane Biophysics: New Insights   and Methods, ‎Springer2018, pp   147-173.（受邀撰写章节）.

2.            Hua He, Huifeng Qian, Chaoqing   Dong, Kanglin Wang, Jicun Ren*. Single nonblinking CdTe quantum dots   synthesized in aqueous thiopropionic acid. Angew. Chem. Int. Ed.2006, 45(45): 7588-7591. (一区，IF=12.102, 被Nat. Mater.,   Phys. Rev. Lett.等顶尖期刊正面引用84次）

3.            Hua He, Xu Liu, Shan Li, Xiaojuan   Wang, Qian Wang, Jiqiang Li, Junying Wang, Hao Ren, Baosheng Ge, Shengjie   Wang, Xiaodong Zhang, Fang Huang, High-Density Super-Resolution Localization   Imaging with Blinking Carbon Dots. Anal.   Chem.2017, 89 (21), 11831-11838.（一区，IF=6.042, 被顶尖期刊Nat. Meth.亮点引用）

4.            Hua He, Chao Xie, Jicun Ren*.   Non-Bleaching Fluorescence of Gold Nanoparticles and Its Applications in   Cancer Cell Imaging. Anal. Chem.2008, 80(15): 5951-5957.（一区，IF=6.042,   被JACS等期刊引用206次）

5.            Naseer Ullah Khan, Hua He*（通讯作者）, Xiaojuan Wang, Baosheng Ge, Qian Wang, Xu Liu, Jun Lao, Yanan Wang,   Jiqiang Li, Zhencai Wang, Shitan Zhou, and Fang Huang*. A Two-Color   Fluorescence Enhanced Dot-Blot Assay for Revealing Co-operative Expression of   Chemokine Receptors in Cells. Chem.   Commun. 2018, 54, 778-781.（一区，IF=6.29, TOP期刊）

6.            Hua He, Zhencai Wang, Tiantian   Cheng, Xu Liu, Xiaojuan Wang, Junying Wang, Hao Ren, Yawei Sun, Yanzhuo Song,   Jiang Yang, Yongqing Xia, Shengjie Wang, Xiaodong Zhang*, and Fang Huang*.   Visible and Near-Infrared Dual-Emission Carbogenic Small Molecular Complex   with High RNA Selectivity and Renal Clearance for Nucleolus and Tumor   Imaging. ACS Appl. Mater. Interfaces   2016, 8 (42), 28529-28537.（一区，IF=8.097，TOP期刊）

7.            Hua He, Min Feng, Jing Hu, Cuixia   Chen, Jiqian Wang, Xiaojuan Wang, Hai Xu*, Jian R. Lu*. Designed Short RGD   Peptides for One-Pot Aqueous Synthesis of Integrin-binding CdTe and CdZnTe   Quantum Dots. ACS Appl. Mater.   Interfaces2012, 4, 6362-6370.（一区，IF=8.097，TOP期刊）

8.            Hua He, Xiaojuan Wang, Tiantian   Cheng, Yongqing Xia, Jun Lao, Baosheng Ge, Hao Ren, Naseer Ullah Khan, Fang   Huang*. An Ultra-High Fluorescence Enhancement and High Throughput Assay for   Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chem. Eur. J.2016, 22,   5863-5867.（二区，IF=5.16，TOP期刊）

9.            Hua He*, Xiaojuan Wang, Zhenzhen   Feng, Tiantian Cheng, Xing Sun, Yawei Sun, Yongqing Xia, Shengjie Wang,   Junying Wang, Xiaodong Zhang*. Rapid microwave-assisted synthesis of   ultra-bright fluorescent carbon dots for live cell staining, cell-specific   targeting and in vivo imaging. J.   Mater. Chem. B2015, 3, 4786-4789.（一区，IF=4.776，TOP期刊）

10.         Naseer Ullah Khan, Zhenzhen Feng, Hua He*（通讯作者）, Qian Wang, Xu Liu, Shan Li, Xinjian Shi, Xiaojuan Wang, Baosheng Ge   and Fang Huang*, A Facile Plasmonic Silver Needle for Fluorescence-Enhanced   Detection of Tumor Markers. Anal.   Chim. Acta. 2018, 1040, 120-127(二区，IF=5.123, TOP期刊）

11.         Hua He, Shan Li, Xinjian Shi,   Xiaojuan Wang, Xu Liu, Qian Wang, Aijun Guo, Baosheng Ge, Naseer Ullah Khan,   and Fang Huang, Quantitative Nanoscopy of Small Blinking Graphene   Nanocarriers in Drug Delivery. Bioconjugate   Chem. 2018, 29 (11), 3658–3666. (二区，IF=4.485).

12.         Hua He, Jicun Ren*. A Novel   Evanescent Wave Scattering Imaging Method for Single Gold Particle Tracking   in Solution and on Cell Membrane. Talanta2008, 77(1):166-171.（二区，IF=4.244）

13.         Hua He, Qingji Xie, Shouzhuo Yao*.   An electrochemical quartz crystal impedance study on anti-human   immunoglobulin G immobilization in the polymer grown during dopamine   oxidation at an Au electrode. J.   Colloid Interf. Sci.2005, 289, 446-454.（二区，IF=5.091）

14.         Jun Lao, Hua He (共同第一作者), Xiaojuan Wang, Zhencai   Wang, Yanzhuo Song, Bin Yang, Naseer Ullahkhan, Baosheng Ge*, Fang Huang*.   Single-Molecule Imaging Demonstrates Ligand Regulation of Oligomeric Status   of CXCR4 in Living Cells. ‎J. Phys. Chem. B 2017, 121 (7), 1466-1474.（三区，IF=3.146）

15.         Lingfang Liu, Xiaoyu Mu, Haixia Liu, Qian Wang, Xueting Bai, Junying   Wang , Haile Liu , Fujuan Xu, Yaqi Jing, Haitao Dai, Changlong Liu*, Hua   He*(通讯作者), and Xiao-Dong Zhang *, Structure, luminescence, and bioimaging of 2   bimetallic CuAu nanoclusters. Opt.   Mater.2018, 86, 291–297.（三区，IF=2.32）

16.         Hua He, Xing Sun, Xiaojuan Wang,   Yawei, Sun, Hai Xu*, Jian R. Lu*. Cyclic Arginyl-Glycyl-Aspartic Acid (RGD)   Peptide-Induced Synthesis of Uniform and Stable One-Dimensional CdTe   Nanostructures in Aqueous Solution. RSC   Adv.2014, 4, 11794-11797.（三区，IF=2.936）

17.         Yuan He, Zhenzhen Feng, Xinjian Shi, Shan Li, Yunguo, Liu, Guangming   Zeng, and Hua He*(通讯作者), N- and O-doped Carbon Dots for Rapid and High-Throughput Dual   Detection of Trace Amounts of Iron in Water and Organic Phases. J. Fluoresc. 2018, DOI:   10.1007/s10895-018-2321-5.

18.         Hua He*, Xing Sun, Xiaojuan Wang and Hai Xu*,   Synthesis of Highly Luminescent and Biocompatible CdTe/CdS/ZnS Quantum Dots   Using Microwave Irradiation: a Comparative Study of Different Ligands, Luminescence 2014, 29, 837–845.（IF=1.671）

19.         Hua He, Qingji Xie, Youyu Zhang,   Shouzhuo Yao*. A simultaneous electrochemical impedance and quartz crystal   microbalance study on antihuman immunoglobulin G adsorption and human   immunogloulin G reaction. J.   Biochem. Bioph. Meth.2005, 62, 191-205.

◎参与发表的SCI论文：

20.         Xiaojuan Wang, Hua He, Yanan Wang, Junying Wang, Xing   Sun, Hai Xu, Werner M Nau*, Xiaodong Zhang*, Fang Huang*. Active Tumor‐Targeting Luminescent Gold   Clusters with Efficient Urinary Excretion. Chem. Commun.2016, 52, 9232-9235.

21.         Xiaojuan Wang, Yanan Wang, Hua He, Xiqi Ma, Qi Chen,   Shuai Zhang, Baosheng Ge, Shengjie Wang, Werner M Nau, Fang Huang. Deep-Red   Fluorescent Gold Nanoclusters for Nucleoli Staining: Real-Time Monitoring of   the Nucleolar Dynamics in Reverse Transformation of Malignant Cells. ACS Appl. Mater. Interfaces2017,   9 (21), 17799–17806

22.         Xiaojuan Wang, Yanan Wang, Hua He, Xin Chen, Xing Sun,   Yawei Sun, Guangjun Zhou, Hai Xu, Fang Huang*. Steering graphene quantum dots   in living cells: lighting up the nucleolus. J. Mater. Chem. B 2016, 4, 779-784.

23.         Hao Wang, Xiaoyu Mu, Hua He, Xiao-Dong Zhang,Cancer Radiosensitizers, Trends   in Pharmacological Science, 2018, 39, 24-48. (Feature Review).

24.         Xiaojuan Wang, Xing Sun, Hua He, Hao Yang, Jun Lao,   Yanzhuo Song, Yongqing Xia, Hai Xu, Xiaodong Zhang, Fang Huang*. A   two-component active targeting theranostic agent based on graphene quantum   dots. J. Mater. Chem. B2015,   17, 3583-3590.

25.         Xiaodong Zhang, Jie Chen, Jiang Yang, Jun-Ying Wang, Xiu Shen, ShaSha   Song, Hao Wang, Hua He, Xiaojuan Wang, Saijun Fan, Yuanming   Sun, Meili Guo. Use of epidermal growth factor receptor antibody–gold cluster   conjugates with good renal excretion in targeted cancer radiation treatment. J. Mater. Chem. B2015, 3,   4735-4741.

26.         Xiaojuan Wang, Xing Sun, Jun Lao, Hua He, Tiantian Cheng,   Mingqing Wang, Shengjie Wang, Fang Huang*. Multifunctional graphene quantum   dots for simultaneous targeted cellular imaging and drug delivery. Colloids Surf., B2014,   122, 638-644.

27.         Xiaojuan Wang, Qi Chen, Zhengyuan Zhang, Hua He, Xiqi Ma,   Zhenzhen Liu, Baosheng Ge, Fang Huang, Novel Enteromorpha Prolifera based   carbon dots: Probing the radical scavenging of natural phenolic compounds.Colloids Surf., B2018, 174:161-167.

28.         Yongqing Xia, Xinlong He, Meiwen Cao, Xiaojuan Wang, Yawei Sun, Hua   He, Hai Xu*, Jian Ren Lu*. Self-Assembled Two-Dimensional   Thermoresponsive Microgel Arrays for Cell Growth/Detachment Control. Biomacromolecules2014, 15,   4021-4031.

29.         Wei Long, Junying Wang, Jiang Yang, Hongying Wu, Jingya Wang, Xiaoyu   Mu, Hua He, Qiang Liu, Yuanming Sun, Haichao Wang, and Xiaodong Zhang.   Naturally-Derived PHA-L Protein Nanoparticle as a Radioprotector Through   Activation of Toll-Like Receptor 5. J   Biomed. Nanotechnol.2019, 15(1):62-76.

30.         Yanzhuo Song, Baosheng Ge, Jun Lao, Zhencai Wang, Bin Yang, Xiaojuan   Wang, Hua He, Jiqiang Li, Fang Huang, Regulation of the   Oligomeric Status of CCR3 with Binding Ligands Revealed by Single-Molecule   Fluorescence Imaging, Biochemistry2018, 57 (5), 852-860.