王晓娟（副教授）

  姓    名：

王晓娟

  职    称：

副教授

  导师类别：

　硕士生导师

  系    室：

应用化学系

  研究领域：

　功能性化学/生物分子的偶联、组装及应用

  电子邮箱：

xwang@upc.edu.cn

  联系电话：

　（0532）86981560

◎教育背景

1991-1995， 山东大学化学系，理学学士

2000-2004， 瑞士巴塞尔大学 (University of Basel) 化学系，理学博士

◎工作经历

2005-2009， 英国剑桥大学 (Cambridge University) 化学工程和生物技术系，博士后研究员

2009-2011， 英国科技公司  Nanoco Technologies Ltd (Manchester)，研究科学家

2011-至今， 中国石油大学（华东）化学化工学院，副教授

◎研究方向

[1] 依据污染物光降解、发光器件、化学分析、疾病诊疗等应用方向设计纳米粒子的表面修饰并加以优化。

[2] 探究包括有机高分子、生物大分子、无机离子在内的各种分子与纳米粒子的相互作用机理及其应用。

◎科研项目

[1] 海藻基碳纳米复合光催化剂用于土壤内多氯联苯类污染物降解处理的应用效果评价；

[2] 智能型短肽修饰荧光金纳米簇的构建及其标记细胞核仁与载运核酸片段的功能开发；

[3] 石墨烯量子点对分子伴侣介导蛋白质折叠过程的影响及作用机理；

[4] 细胞膜上G蛋白偶联受体聚集与分布的荧光闪烁碳点超分辨成像；

[5]  用于重大恶性肿瘤早期预警的等离子体纳米镀膜检测芯片关键技术；

[6] 利用多肽修饰的无镉量子点作为荧光探针研究CXCR4在细胞膜上的运动；

[7] 无镉量子点在发光二极管技术中的应用。

◎代表性论文及专利

1.论文

[1]X. Wang, Z. Zhang, Z. Liu, X. Ma, Q. Dai, X. Wang, B. Ge, H. He, F. Huang. Spectroscopic investigation on the binding interactions between graphene quantum dots and carbonic anhydrase. Spectrochim Acta, Part A. 2022;265:120369.

[2]刘彩, 刘芳, 黄方, 王晓娟. 海藻基cds-cu-tio₂复合材料的制备及其光催化性能. 无机材料学报. 2021;36(11):1154-1162.

[3]Q. Wang, H. He, Q. Zhang, Z. Feng, J. Li, X. Chen, L. Liu, X. Wang, B. Ge, D. Yu, H. Ren, F. Huang. Deep-learning-assisted single-molecule tracking on a live cell membrane. Anal Chem. 2021;93(25):8810-8816.

[4]Q. Wang, Z. Feng, H. He, X. Hu, J. Mao, X. Chen, L. Liu, X. Wei, D. Liu, S. Bi, X. Wang, B. Ge, D. Yu, F. Huang. Nonblinking carbon dots for imaging and tracking receptors on a live cell membrane. Chem Commun. 2021;57(45):5554-5557.

[5]X. Ma, X. Wang, C. Liu, B. Ge, H. He, Q. Dai, Z. Zhang, J. Yu, W.M. Nau, F. Huang. Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential. Materials & Design. 2021;212:110196.

[6]J. Li, Y. Ding, H. Liu, H. He, D. Yu, X. Wang, X. Wang, X. Yu, B. Ge, F. Huang. Oligomerization-enhanced receptor-ligand binding revealed by dual-color simultaneous tracking on living cell membranes. The journal of physical chemistry letters. 2021;12:8164-8169.

[7]H. He, L. Liu, X. Chen, Q. Wang, X. Wang, W.M. Nau, F. Huang. Carbon dot blinking enables accurate molecular counting at nanoscale resolution. Anal Chem. 2021;93(8):3968-3975.

[8]Q. Dai, X. Wang, C. Liu, Z. Feng, B. Ge, X. Ma, Z. Zhang, J. Yu, X. Wang, F. Huang. Detection of tyrosinase in living cells using an enteromorpha prolifera based fluorescent probe. Anal Chim Acta. 2021;1169:338605.

[9]Y. Xia, S. Gao, H. He, X. Wang, Z. Zeng, B. Chen, N. Zou, M. Cao, S. Wang. A new and straightforward strategy to prepare an optical hydrogel film with dynamic structural colors. The Journal of Physical Chemistry C. 2020;124(29):16083-16089.

[10]X. Ma, X. Wang, Q. Chen, H. He, Y. Sun, H. Liu, Y. Wang, J. Qu, F. Huang. Glycosaminoglycan / gold nanocluster hybrid nanoparticles as a new sensing platform: Metastatic potential assessment of cancer cells. Carbohydr Polym. 2020;230:115654.

[11]H. He, X. Shi, J. Wang, X. Wang, Q. Wang, D. Yu, B. Ge, X. Zhang, F. Huang. Reactive oxygen species-induced aggregation of nanozymes for neuron injury. ACS Appl Mater Interfaces. 2020;12(1):209-216.

[12]Y. Wang, X. Wang, X. Ma, Q. Chen, H. He, W.M. Nau, F. Huang. Coassembly of gold nanoclusters with nucleic acids: Sensing, bioimaging, and gene transfection. Part Part Syst Charact. 2019;36(10):1900281.

[13]X. Wang, Z. Liu, Q. Chen, X. Wang, F. Huang. Interactions between graphene materials and proteins. Progress in Chemistry. 2019;31(2-3):236-244.

[14]X. Wang, Q. Chen, Z. Zhang, H. He, X. Ma, Z. Liu, B. Ge, F. Huang. Novel enteromorpha prolifera based carbon dots: Probing the radical scavenging of natural phenolic compounds. Colloids Surf, B. 2019;174:161-167.

[15]S. Wang, B. Cui, Q. Cai, Y. Bu, X. Wang, M. Cao, Y. Xia, H. He. Fabrication of highly luminescent sio2–au nanostructures and their application in detection of trace hg2+. J Mater Sci. 2019;54(10):7517-7528.

[16]G. Jiang, X. Li, Y. Che, Y. Lv, F. Liu, Y. Wang, C. Zhao, X. Wang. Antibacterial and anticorrosive properties of cuzno@rgo waterborne polyurethane coating in circulating cooling water. Environmental Science and Pollution Research. 2019;26(9):9027-9040.

[17]Y. Xia, D. Tang, H. Wu, X. Wang, M. Cao, H. He, S. Wang. Cell attachment/detachment behavior on poly(n-isopropylacrylamide)-based microgel films: The effect of microgel structure and swelling ratio. J Mater Sci. 2018;53(12):8795-8806.

[18]Y. Song, B. Ge, J. Lao, Z. Wang, B. Yang, X. Wang, H. He, J. Li, F. Huang. Regulation of the oligomeric status of ccr3 with binding ligands revealed by single-molecule fluorescence imaging. Biochemistry (Mosc). 2018;57(5):852-860.

[19]N.U. Khan, H. He, X. Wang, B. Ge, Q. Wang, X. Liu, J. Lao, Y. Wang, J. Li, Z. Wang, S. Zhou, F. Huang. A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells. Chem Commun. 2018;54(7):778-781.

[20]N.U. Khan, Z. Feng, H. He, Q. Wang, X. Liu, S. Li, X. Shi, X. Wang, B. Ge, F. Huang. A facile plasmonic silver needle for fluorescence-enhanced detection of tumor markers. Anal Chim Acta. 2018;1040:120-127.

[21]M.H. Jacob, R.N. D'souza, T. Schwarzlose, X. Wang, F. Huang, E. Haas, W.M. Nau. Method-unifying view of loop-formation kinetics in peptide and protein folding. J Phys Chem B. 2018;122(16):4445-4456.

[22]H. He, X. Wang, F. Huang. Analysis and applications of single-molecule fluorescence in live cell membranes. In: Wang H, Li G, editors. Membrane biophysics: Springer; 2018. p. 147-173.

[23]H. He, S. Li, X. Shi, X. Wang, X. Liu, Q. Wang, A. Guo, B. Ge, N.U. Khan, F. Huang. Quantitative nanoscopy of small blinking graphene nanocarriers in drug delivery. Bioconjug Chem. 2018;29(11):3658-3666.

[24]H. Chi, B. Xu, Z. Liu, J. Wei, S. Li, H. Ren, Y. Xu, X. Lu, X. Wang, X. Wang, F. Huang. Combined thermodynamic and kinetic analysis of groel interacting with cxcr4 transmembrane peptides. Biochim Biophys Acta. 2018;1862:1576-1583.

[25]Y. Xia, Y. Tang, H. Wu, J. Zhang, Z. Li, F. Pan, S. Wang, X. Wang, H. Xu, J.R. Lu. Fabrication of patterned thermoresponsive microgel strips on cell-adherent background and their application for cell sheet recovery. ACS Appl Mater Interfaces. 2017;9(2):1255-1262.

[26]X. Wang, Y. Wang, H. He, X. Ma, Q. Chen, S. Zhang, B. Ge, S. Wang, W.M. Nau, F. Huang. Deep-red fluorescent gold nanoclusters for nucleoli staining: Real-time monitoring of the nucleolar dynamics in reverse transformation of malignant cells. ACS Appl Mater Interfaces. 2017;9(21):17799-17806.

[27]J.-B. Qu, Y.-L. Xu, Y. Liu, Y. Wang, Y. Sui, J.-G. Liu, X. Wang. Inherently fluorescent polystyrene microspheres for coating, sensing and cellular imaging. Colloids Surf, B. 2017;152:475-481.

[28]J. Lao, H. He, X. Wang, Z. Wang, Y. Song, B. Yang, N. Ullahkhan, B. Ge, F. Huang. Single-molecule imaging demonstrates ligand regulation of the oligomeric status of cxcr4 in living cells. J Phys Chem B. 2017;121(7):1466-1474.

[29]H. He, X. Liu, S. Li, X. Wang, Q. Wang, J. Li, J. Wang, H. Ren, B. Ge, S. Wang, X. Zhang, F. Huang. High-density super-resolution localization imaging with blinking carbon dots. Anal Chem. 2017;89(21):11831-11838.

[30]P. Zhao, M. Yang, W. Fan, X. Wang, F. Tang, C. Yang, X. Dou, S. Li, Y. Wang, Y. Cao. Facile one-pot conversion of petroleum asphaltene to high quality green fluorescent graphene quantum dots and their application in cell imaging. Part Part Syst Charact. 2016;33(9):635-644.

[31]Y. Xu, J. Qu, X. Wang, J. Liu, S. Li, J. Liu. Preparation of polystyrene-based fluorescent microspheres and their luminescence mechanism. Acta Polym Sin. 2016(10):1368-1373.

[32]X. Wang, Y. Wang, H. He, X. Chen, X. Sun, Y. Sun, G. Zhou, H. Xu, F. Huang. Steering graphene quantum dots in living cells: Lighting up the nucleolus. J Mater Chem B. 2016;4(4):779-784.

[33]X. Wang, H. He, Y. Wang, J. Wang, X. Sun, H. Xu, W.M. Nau, X. Zhang, F. Huang. Active tumor-targeting luminescent gold clusters with efficient urinary excretion. Chem Commun. 2016;52(59):9232-9235.

[34]H. He, Z. Wang, T. Cheng, X. Liu, X. Wang, J. Wang, H. Ren, Y. Sun, Y. Song, J. Yang, Y. Xia, S. Wang, X. Zhang, F. Huang. Visible and near-infrared dual-emission carbogenic small molecular complex with high rna selectivity and renal clearance for nucleolus and tumor imaging. ACS Appl Mater Interfaces. 2016;8(42):28529-28537.

[35]H. He, X. Wang, T. Cheng, Y. Xia, J. Lao, B. Ge, H. Ren, N.U. Khan, F. Huang. An ultra-high fluorescence enhancement and high throughput assay for revealing expression and internalization of chemokine receptor cxcr4. Chem--Eur J. 2016;22(17):5863-5867.

[36]X.-D. Zhang, J. Chen, J. Yang, J.-Y. Wang, X. Shen, S.-S. Song, H. Wang, H. He, X. Wang, S. Fan, Y.-M. Sun, M. Guo. Use of epidermal growth factor receptor antibody-gold cluster conjugates with good renal excretion in targeted cancer radiation treatment. J Mater Chem B. 2015;3(23):4735-4741.

[37]T. Yue, X. Wang, X. Zhang, F. Huang. Molecular modeling of interaction between lipid monolayer and graphene nanosheets: Implications for pulmonary nanotoxicity and pulmonary drug delivery. RSC Adv. 2015;5(38):30092-30106.

[38]X. Wang, X. Sun, H. He, H. Yang, J. Lao, Y. Song, Y. Xia, H. Xu, X. Zhang, F. Huang. A two-component active targeting theranostic agent based on graphene quantum dots. J Mater Chem B. 2015;3:3583-3590.

2.专利

[1] 王晓娟，黄方，王雅楠，曲剑波，何化，王生杰，一种KCK多肽修饰的金纳米簇及其制备方法， 201610387321.0

[2] 王晓娟，曲剑波，王雅楠，黄方，何化，王生杰，一种多肽修饰的金纳米簇及其制备方法， 201610389189.7

[3] 王晓娟，陈奇，张政媛，黄方，王生杰，潘思远，葛保胜，何化，一种以海藻为碳源的荧光碳量子点的制备方法和用途， 201611128316.4

[4] 王晓娟，马锡琦，王雅楠，黄方，葛保胜，何化，一种基因载体系统及其构建方法， 201711350421.7

[5] 王晓娟，陈奇，黄方，葛保胜，刘真真，一种以碳量子点为荧光指示剂的酚类抗氧化剂测定方法， 201711350390.5

[6] 王生杰，王晓娟，荆倩，曹美文，夏永清，一种石墨烯分散液的制备方法，201711379802.8

[7] 王晓娟，马锡琦，黄方，葛保胜，孙怡鑫，一种检测肝素钠中多硫酸软骨素类杂质的方法， 201711430491.3

[8] 王晓娟,戴祺，孙怡鑫，曲剑波，黄方，张智雄，一种基于碳量子点构建的酪氨酸酶检测探针及其制备方法和应用，201911162539.6

[9] 王晓娟，于锦艺，刘慧，黄方，戴祺，一种多功能脂质体的制备方法，202010709982.7

[10] 王晓娟，张智雄，马锡琦，维尔纳•M•诺，黄方，戴祺，一种蛋白质胞内递送的方法，202010960117.X

[11] 王晓娟，刘彩，刘芳，黄方，葛保胜，戴祺，一种仿生合成活性炭-二氧化钛复合材料的制备方法，202011203083.6

◎执教课程

本科生：《现代仪器技术》《生物物理化学（双语）》《专业外语》《化学与服饰》

研究生、留学生：《高等生物化学》《生物仪器技术》

◎招收及指导研究生情况

1.指导研究生情况

协助指导博士研究生1名，学术研究生17名，专业硕士研究生6名。

毕业研究生8名，其中2名出国攻读博士学位，3名到机关/学校任职，3名到科技公司承担研发工作。

2.典型学生：

2012级研究生孙星，硕士期间发表SCI论文5篇，2014年获得罗伯托罗卡奖学金，2020年在德国 Marburg University 获得博士学位之后回国在湖南大学工作；

2014级研究生王雅楠，硕士期间发表SCI论文5篇，2016年获得国家奖学金，2017年获得CSC奖学金赴瑞士 University of Geneva攻读博士学位，2022年赴美国密歇根大学继续博士后研究。

2019级研究生戴祺，硕士期间已经发表SCI论文3篇（另有3篇在投），2021年获得国家奖学金，即将去四川大学继续攻读博士学位。

3.招生专业及要求

学术学位硕士生：081700化学工程与技术；专业学位硕士生：085602化学工程